| Registro completo |
| Provedor de dados: |
Genet. Mol. Biol.
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| País: |
Brazil
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| Título: |
Co-transformation of a tropical maize endophytic isolate of Fusarium verticillioides (synonym F. moniliforme) with gusA and nia genes
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| Autores: |
Pamphile,João A.
Rocha,Carmen Lúcia M.S.C. da
Azevedo,João L.
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| Data: |
2004-01-01
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| Ano: |
2004
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| Palavras-chave: |
Co-transformation
Gus
Nia
Fusarium verticillioides
Endophyte
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| Resumo: |
A tropical endophytic isolate of the fungus Fusarium verticillioides (synonym Fusarium moniliforme) obtained from Zea mays was co-transformed with plasmid pNH24 containing the Fusarium oxysporum nitrate reductase nia gene and plasmid pNOM 102 carrying the Escherichia coli b-glucuronidase gusA gene. Transformation frequency for the nia marker was 75 transformants mg-1 vector DNA and introduction of the gusA gene by co-transformation was 57.2% as indicated by the presence of the GUS+ phenotype on plates. Southern analyses confirmed the integration of both plasmids into the genome of ten GUS+ transformants. All co-transformants showed mitotic stability in respect of the GUS+ phenotype. To assess the potential of transformed endophytic fungi as vectors for introducing desirable characteristics into host tropical plants of biotechnological and agricultural importance we successfully infected maize roots and detected GUS+ phenotype.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200021
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| Editor: |
Sociedade Brasileira de Genética
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| Relação: |
10.1590/S1415-47572004000200021
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| Formato: |
text/html
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| Fonte: |
Genetics and Molecular Biology v.27 n.2 2004
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| Direitos: |
info:eu-repo/semantics/openAccess
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